Semester 8

1. Familiarization with Plant Tissue Culture Laboratory facilities, keeping of records, accounts of plant tissue culture, Collection of literature on available micropropagation protocols, quality assurance of TC plants – NCS-TCP. 2. Market survey, financial implications of commercial tissue culture facility, collection of details on cost of lab equipment, chemicals, glass wares and other required items, preparation of work plan 3. Procurement of chemicals, glass wares, disinfectants etc. disinfection and sterilization of glass wares, collection and maintenance of explant source 4. Preparation of stock solutions for tissue culture media, plant tissue culture media preparation and sterilization 5. Culture establishment of banana and virus indexing of established sprouts 6. Culture establishment of orchids and anthurium 7. Subculturing of established cultures 8. Subculturing of established cultures 9. Propagule multiplication in banana/orchids/ anthurium 10. Propagule multiplication in banana/orchids/ anthurium 11. Propagule multiplication in banana/orchids/ anthurium 12. Propagule multiplication in banana/orchids/ anthurium 13. Propagule multiplication in banana/orchids/ anthurium 14. Propagule multiplication in banana/orchids/ anthurium 15. Induction of rooting 16. Induction of rooting 17. Hardening of in vitro regenerated plantlets 18. Hardening of in vitro regenerated plantlets 19. Hardening of in vitro regenerated plantlets 20. Clonal fidelity analysis 21. Virus indexing of 0.1 per cent regenerated plantlets of banana 22. Marketing of TC plants 23. Visit to commercial micropropagation unit. 24. Report submission, Evaluation,Practical Examination

Practical schedule (24 weeks) 1. Familiarization with Plant Tissue Culture Laboratory facilities, keeping of records, accounts of plant tissue culture, Collection of literature on available micropropagation protocols, quality assurance of TC plants – NCS-TCP. 2. Market survey, financial implications of commercial tissue culture facility, collection of details on cost of lab equipment, chemicals, glass wares and other required items, preparation of work plan 3. Procurement of chemicals, glass wares, disinfectants etc. disinfection and sterilization of glass wares, collection and maintenance of explant source 4. Preparation of stock solutions for tissue culture media, plant tissue culture media preparation and sterilization 5. Culture establishment of banana and virus indexing of established sprouts 6. Culture establishment of orchids and anthurium 7. Subculturing of established cultures 8. Subculturing of established cultures 9. Propagule multiplication in banana/orchids/ anthurium 10. Propagule multiplication in banana/orchids/ anthurium 11. Propagule multiplication in banana/orchids/ anthurium 12. Propagule multiplication in banana/orchids/ anthurium 13. Propagule multiplication in banana/orchids/ anthurium 14. Propagule multiplication in banana/orchids/ anthurium 15. Induction of rooting 16. Induction of rooting 17. Hardening of in vitro regenerated plantlets 18. Hardening of in vitro regenerated plantlets 19. Hardening of in vitro regenerated plantlets 20. Clonal fidelity analysis 21. Virus indexing of 0.1 per cent regenerated plantlets of banana 22. Marketing of TC plants 23. Visit to commercial micropropagation unit. 24. Report submission, Evaluation,Practical Examination

Course teachers: Dr. A. Sakeer Husain and Dr. M.J. Mercykkutty

Dept. of Agrl. Extension, College of Horticulture, Vellanikkara

Introduction to course, familiarisation with nursery activities, records and registers and preparation of inventory, familiarisation with growing media components

preparation of different types of cuttings

preparation of different types of layers

practising commercial propagation methods like grafting, budding and layering 

The Department of Agronomy, COA, Vellanikkara  is offering a (0+10) course Elcp 4205 for the final year BSc (Ag) students as a part of Experiential learning programme  under the STUDENT READY programme as envisaged by ICAR. About thirteen topics under Organic production technology including preparation of various organic manures, bio fertilizers , studies on bio control agents, mass production of enriched composts , raising organic vegetables in open terraces and  poly houses are also being focused. Apart from these, classes on organic certification and marketing aspects are also covered. Finally to cap it all, a project proposal and work plan for establishing an organic farm and manure production unit is also planned to develop the entrepreneurial instincts of the students. Under the present situation in the wake of Covid pandemic, it is proposed that possible theory topics can be covered under online mode but the major practical works may be carried out in the field itself. Smt Raji S Prasad , Smt Jayasree P and Smt Anjana Babu S R , Assistant Professors , Agronomy are assigned the duties and responsibilities in connection with the course work under the leadership of the HOD i/c Dr. P. Prameela. For undertaking the various practical works under this course several farm inputs are required.  Inputs shall be procured from the various production units under KAU, farmers, and other registered input agencies.