1. Familiarization with Plant Tissue Culture Laboratory facilities, keeping of records, accounts
of plant tissue culture, Collection of literature on available micropropagation protocols,
quality assurance of TC plants – NCS-TCP.
2. Market survey, financial implications of commercial tissue culture facility, collection of
details on cost of lab equipment, chemicals, glass wares and other required items, preparation
of work plan
3. Procurement of chemicals, glass wares, disinfectants etc. disinfection and sterilization of glass
wares, collection and maintenance of explant source
4. Preparation of stock solutions for tissue culture media, plant tissue culture media preparation
and sterilization
5. Culture establishment of banana and virus indexing of established sprouts
6. Culture establishment of orchids and anthurium
7. Subculturing of established cultures
8. Subculturing of established cultures
9. Propagule multiplication in banana/orchids/ anthurium
10. Propagule multiplication in banana/orchids/ anthurium
11. Propagule multiplication in banana/orchids/ anthurium
12. Propagule multiplication in banana/orchids/ anthurium
13. Propagule multiplication in banana/orchids/ anthurium
14. Propagule multiplication in banana/orchids/ anthurium
15. Induction of rooting
16. Induction of rooting
17. Hardening of in vitro regenerated plantlets
18. Hardening of in vitro regenerated plantlets
19. Hardening of in vitro regenerated plantlets
20. Clonal fidelity analysis
21. Virus indexing of 0.1 per cent regenerated plantlets of banana
22. Marketing of TC plants
23. Visit to commercial micropropagation unit.
24. Report submission, Evaluation,Practical Examination
Practical schedule (24 weeks)
1. Familiarization with Plant Tissue Culture Laboratory facilities, keeping of records, accounts
of plant tissue culture, Collection of literature on available micropropagation protocols,
quality assurance of TC plants – NCS-TCP.
2. Market survey, financial implications of commercial tissue culture facility, collection of
details on cost of lab equipment, chemicals, glass wares and other required items, preparation
of work plan
3. Procurement of chemicals, glass wares, disinfectants etc. disinfection and sterilization of glass
wares, collection and maintenance of explant source
4. Preparation of stock solutions for tissue culture media, plant tissue culture media preparation
and sterilization
5. Culture establishment of banana and virus indexing of established sprouts
6. Culture establishment of orchids and anthurium
7. Subculturing of established cultures
8. Subculturing of established cultures
9. Propagule multiplication in banana/orchids/ anthurium
10. Propagule multiplication in banana/orchids/ anthurium
11. Propagule multiplication in banana/orchids/ anthurium
12. Propagule multiplication in banana/orchids/ anthurium
13. Propagule multiplication in banana/orchids/ anthurium
14. Propagule multiplication in banana/orchids/ anthurium
15. Induction of rooting
16. Induction of rooting
17. Hardening of in vitro regenerated plantlets
18. Hardening of in vitro regenerated plantlets
19. Hardening of in vitro regenerated plantlets
20. Clonal fidelity analysis
21. Virus indexing of 0.1 per cent regenerated plantlets of banana
22. Marketing of TC plants
23. Visit to commercial micropropagation unit.
24. Report submission, Evaluation,Practical Examination
Course teachers: Dr. A. Sakeer Husain and Dr. M.J. Mercykkutty
Dept. of Agrl. Extension, College of Horticulture, Vellanikkara
Introduction to course, familiarisation with nursery activities, records and registers and preparation of inventory, familiarisation with growing media components
preparation of different types of cuttings
preparation of different types of layers
practising commercial propagation methods like grafting, budding and layering
The
Department of Agronomy, COA, Vellanikkara
is offering a (0+10) course Elcp 4205 for the final year BSc (Ag)
students as a part of Experiential learning programme under the STUDENT READY programme as
envisaged by ICAR. About thirteen topics under Organic production technology
including preparation of various organic manures, bio fertilizers , studies on
bio control agents, mass production of enriched composts , raising organic
vegetables in open terraces and poly
houses are also being focused. Apart from these, classes on organic
certification and marketing aspects are also covered. Finally to cap it all, a
project proposal and work plan for establishing an organic farm and manure
production unit is also planned to develop the entrepreneurial instincts of the
students. Under the present situation in the wake of Covid pandemic, it is proposed
that possible theory topics can be covered under online mode but the major
practical works may be carried out in the field itself. Smt Raji S Prasad , Smt
Jayasree P and Smt Anjana Babu S R , Assistant Professors , Agronomy are
assigned the duties and responsibilities in connection with the course work under the leadership of the HOD i/c Dr. P. Prameela.
For undertaking the various practical works under this course several farm
inputs are required. Inputs shall be
procured from the various production units under KAU, farmers, and other
registered input agencies.